2Z2W
Human Wee1 kinase complexed with inhibitor PF0335770
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2002-06-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.747, 69.747, 157.215 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.920 - 2.220 |
R-factor | 0.21795 |
Rwork | 0.215 |
R-free | 0.27064 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x8b |
RMSD bond length | 0.027 |
RMSD bond angle | 2.433 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.075 | 0.530 |
Number of reflections | 19968 | |
<I/σ(I)> | 46 | 3.5 |
Completeness [%] | 96.8 | 68.9 |
Redundancy | 12.8 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | NaCL, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |