2YZK
Crystal structure of orotate phosphoribosyltransferase from Aeropyrum pernix
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9789, 0.9794, 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.628, 80.552, 118.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.320 - 1.800 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.038 | 0.114 |
| Number of reflections | 100801 | |
| Completeness [%] | 97.1 | 96.5 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 293 | 0.2M Sodium Acetate, 20% PEG3350, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
