2YYY
Crystal structure of Glyceraldehyde-3-phosphate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.117 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 83.398, 152.040, 118.552 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.17069 |
| Rwork | 0.169 |
| R-free | 0.19795 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cf2 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.507 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.066 | 0.238 |
| Number of reflections | 62507 | |
| Completeness [%] | 97.1 | 78.1 |
| Redundancy | 4.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | oil-batch | 8.5 | 293 | 0.2M magnesium chloride hexahydrate, 0.08M tris hydrochloride, pH 8.5, 24%(w/v) PEG4000, 20%(v/v) glycerol anhydrous, oil-batch, temperature 293K |
