2YYU
Crystal structure of uncharacterized conserved protein from Geobacillus kaustophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-26 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.650, 70.151, 62.865 |
| Unit cell angles | 90.00, 106.86, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.760 |
| Number of reflections | 81050 |
| Completeness [%] | 98.0 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES-Na, 10% iso-propanol, 20% PEG 4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
