2YY5
Crystal Structure of tryptophanyl-tRNA synthetase from Mycoplasma pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 88.163, 52.109, 194.092 |
| Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
| Resolution | 34.030 - 2.550 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i6m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 58085 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 0.15M Ammonium Sulfate, 18% PEG3350, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
