2YWB
Crystal structure of GMP synthetase from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-21 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.949, 114.854, 160.033 |
Unit cell angles | 90.00, 93.37, 90.00 |
Refinement procedure
Resolution | 43.280 - 2.100 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.27200 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.066 | 0.411 |
Number of reflections | 145950 | |
Completeness [%] | 98.2 | 94.7 |
Redundancy | 3.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1.4M NaCl, 0.1M Sodium Acetate, pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1.4M NaCl, 0.1M Sodium Acetate, pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1.4M NaCl, 0.1M Sodium Acetate, pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |