2YV6
Crystal structure of human Bcl-2 family protein Bak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-30 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97105 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 61.824, 61.824, 137.482 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.890 - 2.500 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 5908 | |
| <I/σ(I)> | 25.9 | 14.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 22.2 | 23.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | ammonium sulfate, iso-propanol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
