2YRF
Crystal structure of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis complexed with sulfate ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2004-09-27 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 |
Unit cell lengths | 69.273, 69.273, 154.436 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.360 - 2.700 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w2w |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 69.200 |
High resolution limit [Å] | 2.700 |
Number of reflections | 18662 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 50mM MES, 50mM Tris (pH7.4), 1.55M ammonium sulfate, 5% 1,4-dioxane, 6% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |