2YQ6
Structure of Bcl-xL bound to BimSAHB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-06 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.736, 35.336, 61.412 |
| Unit cell angles | 90.00, 100.61, 90.00 |
Refinement procedure
| Resolution | 36.238 - 1.799 |
| R-factor | 0.1682 |
| Rwork | 0.167 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3FDL CHAIN A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.948 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 14372 | |
| <I/σ(I)> | 30.9 | 3.2 |
| Completeness [%] | 98.1 | 85 |
| Redundancy | 6.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15 % (W/V) PEG 400, 5 % PEG 3000, 10 % GLYCEROL AND 0.1 M MES PH 6.5. |
