2YQ5
Crystal Structure of D-isomer specific 2-hydroxyacid dehydrogenase from Lactobacillus delbrueckii ssp. bulgaricus: NAD complexed form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.718, 110.012, 147.736 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.990 - 2.750 |
| R-factor | 0.19122 |
| Rwork | 0.188 |
| R-free | 0.25895 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dxy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.678 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.240 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.100 | 0.560 |
| Number of reflections | 39722 | |
| <I/σ(I)> | 12.7 | 2.6 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG 3350, 200MM MGCL2, 100MM HEPES PH7.5 |
