2YPZ

KSHV LANA (ORF73) C-terminal domain, decameric ring: orthorhombic crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-03
Detector	RIGAKU-MSC SATURN 944 PLUS
Spacegroup name	P 21 21 21
Unit cell lengths	76.790, 100.290, 214.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.427 - 3.199
R-factor	0.2201
Rwork	0.218
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ypy
RMSD bond length	0.003
RMSD bond angle	0.734
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	3.390
High resolution limit [Å]	3.200	3.200
Rmerge	0.190	0.610
Number of reflections	27442
<I/σ(I)>	7.67	2.03
Completeness [%]	97.5	95.1
Redundancy	3.45	3.37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	1.5 UL OF 0.8 MM PROTEIN IN 5 MM BISTRIS, PH 6.5, 200 MM LICL, 4 MM DTT WERE ADDED TO 1.5 UL OF 0.2 M LITHIUM CITRATE, PH 7.6, 20 % (W/V) PEG 3350. THE MIXTURE WAS INCUBATED AT 20 DEGREE CENTIGRADE IN A HANGING DROP SETUP. CRYSTALS GREW IN A FEW DAYS AND WERE CRYO-PROTECTED BY SHORT SOAKING IN 0.2 M LITHIUM CITRATE, PH 7.6, 20 % (W/V) PEG 3350, 30 % (V/V) GLYCEROL.