2YPR
Crystal structure of the DNA binding ETS domain of human protein FEV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 130.770, 130.770, 130.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.500 - 2.640 |
| R-factor | 0.2162 |
| Rwork | 0.216 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fli |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.880 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.500 | 2.770 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.090 | |
| Number of reflections | 11808 | |
| <I/σ(I)> | 19.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.9 | 16.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 14% PEG3350, 5% GLYCEROL, 100 MM CITRATE PH 5.3 |
