2YPN
Hydroxymethylbilane synthase
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID09 |
Synchrotron site | ESRF |
Beamline | ID09 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 1995-11-15 |
Wavelength(s) | 0.4,1.6 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.060, 75.730, 50.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.500 - 2.300 |
Rwork | 0.194 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ah5 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.574 |
Data reduction software | LAUENORM/AGROVATA |
Data scaling software | LAUENORM |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1F) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.500 | 2.390 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.102 | 0.258 |
Number of reflections | 13949 | |
<I/σ(I)> | 5.9 | 2.4 |
Completeness [%] | 89.8 | 89.6 |
Redundancy | 11 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.3 | PROTEIN WAS CRYSTALLISED AT PH 5.3 IN ITTING DROPS OF 0.05ML WITH 6-7MG/ML OF PROTEIN, 0.3MM EDTA, 15MM DITHIOTHREITOL, 10%(W/V) PEG6000 AND 0.01% NAN3 IN 0.1M NAAC. |