2YPL

Structural features underlying T-cell receptor sensitivity to concealed MHC class I micropolymorphisms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	56.348, 75.605, 241.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.770 - 2.400
R-factor	0.22358
Rwork	0.221
R-free	0.27746
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bvo
RMSD bond length	0.007
RMSD bond angle	1.222
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.510
High resolution limit [Å]	2.400	2.400
Rmerge	0.050	0.360
Number of reflections	41023
<I/σ(I)>	40.1	5.5
Completeness [%]	99.0	94.7
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.3		pH 7.3