2YOZ
Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, crystallized with 2'-AMPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-03 |
| Detector | MAR555 FLAT PANEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.400, 47.880, 51.150 |
| Unit cell angles | 90.00, 94.45, 90.00 |
Refinement procedure
| Resolution | 16.999 - 2.100 |
| R-factor | 0.1773 |
| Rwork | 0.175 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmi |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.817 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.100 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.520 |
| Number of reflections | 10896 | |
| <I/σ(I)> | 11.7 | 2 |
| Completeness [%] | 99.4 | 98.6 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 277 | 250 UM PROTEIN AND 10 MM 2, 3(SP)-CYCLIC-AMPS MIXED IN 0.5 PLUS 0.5 DROPS WITH 30% PEG4000 AND 50 MM ACETATE (1:1 MIX OF PH 3 AND PH 5) IN 4C TEMP |
