2YOB
High resolution AGXT_M structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-08 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.495, 103.525, 153.832 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.950 - 1.900 |
R-factor | 0.1713 |
Rwork | 0.170 |
R-free | 0.20430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h0c |
RMSD bond length | 0.007 |
RMSD bond angle | 1.064 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.220 | 2.000 |
High resolution limit [Å] | 1.900 | 1.910 |
Rmerge | 0.100 | 0.540 |
Number of reflections | 69440 | |
<I/σ(I)> | 20.2 | 1.4 |
Completeness [%] | 99.8 | 99 |
Redundancy | 8.7 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 15% PEG 3350, 0.1M BIS-TRIS PH 5.2, 150MM LI2SO4 AND 5% W/V OCTYL-B-D-GLUCOSIDE |