2YMW
Structure of the epsilon-lysine oxidase from Marinomonas mediterranea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-20 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 70.680, 127.883, 106.897 |
| Unit cell angles | 90.00, 107.18, 90.00 |
Refinement procedure
| Resolution | 35.280 - 2.410 |
| R-factor | 0.1963 |
| Rwork | 0.192 |
| R-free | 0.27920 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.280 | 2.540 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.110 | 0.660 |
| Number of reflections | 68875 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 98.8 | 94 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 10% ETHANOL, 0.2 M MAGNESIUM CHLORIDE, 0.1 M IMIDAZOLE 8.0 |
