2YMK
Crystal structure of the hexameric anti-microbial peptide channel dermcidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.354, 50.724, 45.738 |
| Unit cell angles | 90.00, 117.74, 90.00 |
Refinement procedure
| Resolution | 33.790 - 2.490 |
| R-factor | 0.20838 |
| Rwork | 0.205 |
| R-free | 0.27384 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.333 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.020 | 0.510 |
| Number of reflections | 5189 | |
| <I/σ(I)> | 17 | 2.95 |
| Completeness [%] | 98.8 | 96.8 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M ZN(AC)2, 0.1 M NA-CACODYLATE, 18% PEG8000 AT PH 6.5 |
