2YLE
Crystal structure of the human Spir-1 KIND FSI domain in complex with the FSI peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.675, 65.675, 77.964 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.440 - 1.800 |
| R-factor | 0.18884 |
| Rwork | 0.187 |
| R-free | 0.23577 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.226 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.920 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.740 |
| Number of reflections | 16921 | |
| <I/σ(I)> | 12.2 | 2.3 |
| Completeness [%] | 96.2 | 95.6 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
