2YKO
Structure of the human LINE-1 ORF1p trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-06-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.971000, 0.979868 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.690, 85.420, 111.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.824 - 2.100 |
| R-factor | 0.2109 |
| Rwork | 0.208 |
| R-free | 0.26390 |
| Structure solution method | SAD |
| Starting model (for MR) | PDB ENTRIES 2WPQ AND 2W7A |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.273 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.800 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 40087 | |
| <I/σ(I)> | 10.9 | 2 |
| Completeness [%] | 97.2 | 97.6 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 100 MM NA-HEPES PH7.0, 1.1 M NA-MALONATE |
