2YJQ
Structure of a Paenibacillus Polymyxa Xyloglucanase from Glycoside Hydrolase Family 44
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2010-10-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.200, 84.200, 320.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 106.680 - 2.250 |
| R-factor | 0.19988 |
| Rwork | 0.197 |
| R-free | 0.25948 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | APO STRUCTURE PDB ENTRY 2YKK |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.802 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.010 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.100 | 0.560 |
| Number of reflections | 62986 | |
| <I/σ(I)> | 14.9 | 2.7 |
| Completeness [%] | 98.5 | 92.5 |
| Redundancy | 8.1 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% (W/V) PEG 3350, 0.2M LITHIUM SULPHATE, 0.1M BIS TRIS PH 6.5 |
