2YIN
STRUCTURE OF THE COMPLEX BETWEEN Dock2 AND Rac1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.510, 98.610, 130.130 |
| Unit cell angles | 90.00, 99.64, 90.00 |
Refinement procedure
| Resolution | 64.374 - 2.700 |
| R-factor | 0.2059 |
| Rwork | 0.203 |
| R-free | 0.25480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN A OF PDB ENTRY 1GAF AND CHAIN A OF PDB ENTRY 1MH1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.439 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.180 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.610 |
| Number of reflections | 43702 | |
| <I/σ(I)> | 8.7 | 1.5 |
| Completeness [%] | 93.2 | 90.1 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 293 | 0.1 M MES PH 6.5, 12% (W/V) PEG 3350, 10% (V/V) GLYCEROL AND 150 MM NACL. AT 20 C |
