2YIC
Crystal structure of the SucA domain of Mycobacterium smegmatis alpha- ketoglutarate decarboxylase (triclinic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.539, 83.244, 158.610 |
| Unit cell angles | 99.48, 99.06, 101.25 |
Refinement procedure
| Resolution | 39.200 - 1.960 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2XT9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 2.070 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 263780 | |
| <I/σ(I)> | 8.7 | 2.2 |
| Completeness [%] | 95.2 | 91.5 |
| Redundancy | 2.9 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 46.5% MPD, 100 MM HEPES, PH 7.0 |
