2YHG
Ab initio phasing of a nucleoside hydrolase-related hypothetical protein from Saccharophagus degradans that is associated with carbohydrate metabolism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 113 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.580, 61.580, 125.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.730 - 1.080 |
R-factor | 0.13403 |
Rwork | 0.133 |
R-free | 0.15132 |
Structure solution method | DIRECT METHODS |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.526 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | ACORN |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.140 |
High resolution limit [Å] | 1.080 | 1.080 |
Rmerge | 0.060 | 0.390 |
Number of reflections | 173965 | |
<I/σ(I)> | 17.3 | 3.8 |
Completeness [%] | 96.2 | 82.9 |
Redundancy | 9 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 0.2 M NACL, 0.1 M BIS-TRIS, PH 5.5, 25% POLYETHYLENE GLYCOL 3350. |