2YGT
Clostridium perfringens delta-toxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-05 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 112.932, 49.664, 58.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.477 - 2.400 |
| R-factor | 0.1812 |
| Rwork | 0.179 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pvl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.772 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.200 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.150 | 0.690 |
| Number of reflections | 13432 | |
| <I/σ(I)> | 15.8 | 3.5 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 10.4 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | 6.6 MG/ML PROTEIN AGAINST 25-30% PEG 550 25 MM ZNSO4, 100 MM MES-NAOH PH 6.0, AT 293K. |
