2YGP
WIF domain-EGF-like domain 1 Met77Trp of human Wnt inhibitory factor 1 in complex with 1,2-dipalmitoylphosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 50.760, 134.241, 60.193 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.316 - 2.222 |
| R-factor | 0.1875 |
| Rwork | 0.185 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.217 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.320 | 2.280 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.090 | 0.570 |
| Number of reflections | 10433 | |
| <I/σ(I)> | 12.7 | 2.3 |
| Completeness [%] | 99.4 | 92 |
| Redundancy | 6.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.1 M DIAMMONIUM TARTRATE, PH 7.0, 20 MM SPERMIDINE, 0.5 MM CACL2 |
