2YG9
Structure of an unusual 3-Methyladenine DNA Glycosylase II (Alka) from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.311, 51.372, 90.039 |
Unit cell angles | 90.00, 99.71, 90.00 |
Refinement procedure
Resolution | 42.680 - 1.950 |
R-factor | 0.17315 |
Rwork | 0.170 |
R-free | 0.23101 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yg8 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.476 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.680 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.140 | 0.480 |
Number of reflections | 35790 | |
<I/σ(I)> | 8.8 | 3 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 4.1 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1 UL DROPS OF 10 MG/ML PROTEIN MIXED WITH A SOLUTION CONTAINING 1 M LICL2, 0.1 M MES PH 6.0 AND 10% PEG6000 |