2YG8
Structure of an unusual 3-Methyladenine DNA Glycosylase II (Alka) from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.562, 51.297, 90.458 |
Unit cell angles | 90.00, 99.76, 90.00 |
Refinement procedure
Resolution | 49.750 - 2.000 |
R-factor | 0.16054 |
Rwork | 0.158 |
R-free | 0.20315 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.016 |
RMSD bond angle | 1.430 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.750 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.120 | 0.390 |
Number of reflections | 33423 | |
<I/σ(I)> | 14.8 | 5.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.3 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 1 UL DROPS OF 10 MG/ML PROTEIN MIXED WITH A SOLUTION CONTAINING 1 M LICL2, 0.1 M MES PH 6.0 AND 10% PEG6000 |