2YFR
Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-01 |
| Detector | ADSC CCD |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 172.007, 172.007, 114.581 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 121.630 - 1.750 |
| R-factor | 0.15189 |
| Rwork | 0.151 |
| R-free | 0.16958 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.760 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.550 |
| Number of reflections | 85480 | |
| <I/σ(I)> | 12.7 | 3.2 |
| Completeness [%] | 99.0 | 96.5 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 2 M (NH4)2)SO4, 5% (V/V) 2-PROPANOL, pH 6 |
