2YFH
Structure of a Chimeric Glutamate Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 100.102, 113.859, 109.581 |
| Unit cell angles | 116.54, 101.54, 104.09 |
Refinement procedure
| Resolution | 36.524 - 2.695 |
| R-factor | 0.2184 |
| Rwork | 0.215 |
| R-free | 0.28230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bgv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.420 |
| Number of reflections | 108370 | |
| <I/σ(I)> | 10.8 | 1.6 |
| Completeness [%] | 94.1 | 93.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 1.4 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 6.5-8, 10% DIOXANE |
