2YEQ
Structure of PhoD
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-02 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 147.318, 147.318, 347.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 135.640 - 1.930 |
| R-factor | 0.17962 |
| Rwork | 0.178 |
| R-free | 0.20243 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.302 |
| Data reduction software | autoPROC (USING XDS) |
| Data scaling software | autoPROC (USING SCALA) |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.6.0095) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 135.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.490 |
| Number of reflections | 141401 | |
| <I/σ(I)> | 9.6 | 2 |
| Completeness [%] | 99.0 | 96 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 0.1 NA/K PO4 PH 5.8, 52% PEG 200, 0.2M NACL |
