2YD8

Crystal structure of the N-terminal Ig1-2 module of Human Receptor Protein Tyrosine Phosphatase LAR in complex with sucrose octasulphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 32 2 1
Unit cell lengths	78.768, 78.768, 71.907
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.384 - 2.050
R-factor	0.1965
Rwork	0.195
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yd4
RMSD bond length	0.014
RMSD bond angle	1.232
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.100	0.940
Number of reflections	16614
<I/σ(I)>	24.7	2.7
Completeness [%]	100.0	100
Redundancy	9.9	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1 M MES, 30% PEG 6000, PH 6.0 .