2YC4
Intraflagellar Transport Complex 25-27 from Chlamydomonas
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.020, 68.300, 93.780 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 46.848 - 2.800 |
| R-factor | 0.2298 |
| Rwork | 0.225 |
| R-free | 0.31580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tvg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.650 |
| Number of reflections | 15717 | |
| <I/σ(I)> | 14 | 2 |
| Completeness [%] | 99.3 | 92 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 25%PEG 3350 50MM MES PH 6.0 |
