2YBY
Structure of domains 6 and 7 of the mouse complement regulator Factor H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-17 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.620, 34.611, 107.357 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.090 - 1.580 |
| R-factor | 0.1915 |
| Rwork | 0.190 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2W80 CHAIN A |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 |
| Data reduction software | xia2 (RUNNING XDS) |
| Data scaling software | xia2 (RUNNING SCALA) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.790 | 1.590 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.040 | 0.480 |
| Number of reflections | 15809 | |
| <I/σ(I)> | 28.7 | 3.2 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 6.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.2M NACL 0.1M MES PH 6.0 20% PEG 2000 MME |
