2YBX
Crystal Structure of Human Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.040, 98.508, 104.879 |
| Unit cell angles | 90.00, 93.43, 90.00 |
Refinement procedure
| Resolution | 28.370 - 2.560 |
| R-factor | 0.1907 |
| Rwork | 0.188 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bo1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.470 | 2.700 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.090 | 0.680 |
| Number of reflections | 28302 | |
| <I/σ(I)> | 7.9 | 1.1 |
| Completeness [%] | 98.1 | 96.8 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M MAGNESIUM FORMATE, 16% PEG3350, 2 MM BENZYL(1,3,5)TRIPHOSPHATE, pH 7.5 |
