2YBV
STRUCTURE OF RUBISCO FROM THERMOSYNECHOCOCCUS ELONGATUS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-04 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 115.480, 163.470, 163.540 |
Unit cell angles | 90.00, 108.96, 90.00 |
Refinement procedure
Resolution | 39.840 - 2.300 |
R-factor | 0.19763 |
Rwork | 0.196 |
R-free | 0.23167 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rub |
RMSD bond length | 0.022 |
RMSD bond angle | 1.782 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.170 | 0.430 |
Number of reflections | 253864 | |
<I/σ(I)> | 11.11 | 3.8 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7.3 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 5% PEG8000, 20% PEG200, 10% GLYCEROL, 100MM HEPES, PH 7 |