2YBS
JMJD2A COMPLEXED WITH S-2-HYDROXYGLUTARATE AND HISTONE H3K36me3 PEPTIDE (30-41)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-26 |
Detector | RIGAKU SATURN 944 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 100.924, 149.354, 57.271 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.140 - 2.320 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ox0 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.140 | 2.400 |
High resolution limit [Å] | 2.320 | 2.320 |
Rmerge | 0.070 | 0.360 |
Number of reflections | 36569 | |
<I/σ(I)> | 19.69 | 4.26 |
Completeness [%] | 94.9 | 97.2 |
Redundancy | 5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.1 M CITRATE PH 5.5, 4 MM NICL2, 20% PEG 3350, VAPOUR DIFFUSION, SITTING DROP, TEMPERATURE 277K. |