2YBK

JMJD2A COMPLEXED WITH R-2-HYDROXYGLUTARATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-24
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 2
Unit cell lengths	101.127, 149.332, 56.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.070 - 2.400
R-factor	0.203
Rwork	0.203
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ox0
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.070	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	0.810
Number of reflections	32359
<I/σ(I)>	13.74	2
Completeness [%]	94.2	95.3
Redundancy	5.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	0.1M CITRATE PH 5.5, 4MM NICL2, 20% PEG 3350, VAPOUR DIFFUSION, SITTING DROP, TEMPERATURE 277K.