2YBA
Crystal structure of Nurf55 in complex with histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.980, 88.160, 204.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.470 - 2.550 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | UNPUBLISHED STRUCTURE OF NURF55 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.230 | 2.560 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.084 | 0.430 |
| Number of reflections | 32778 | |
| <I/σ(I)> | 15.2 | 2.4 |
| Completeness [%] | 96.8 | 68.4 |
| Redundancy | 5.3 | 2.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM SODIUM CITRATE, PH 5.4; 200 MM AMMONIUM ACETATE; 23% PEG 3350. |
