2YB0
The Crystal Structure of Leishmania major dUTPase in complex deoxyuridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-13 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.186, 109.186, 308.882 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.590 - 2.280 |
| R-factor | 0.211 |
| Rwork | 0.210 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cje |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.250 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.800 | 2.400 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.110 | 0.710 |
| Number of reflections | 98557 | |
| <I/σ(I)> | 17.4 | 3 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 13.5 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M NACL, 0.1 M BIS-TRIS PH 6.5, 1.5 M (NH4)2SO4 |
