2YAN
Crystal structure of the second glutaredoxin domain of human TXNL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.317, 118.317, 58.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.720 - 1.900 |
| R-factor | 0.18629 |
| Rwork | 0.185 |
| R-free | 0.21237 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1WIK 2wci 2wul 2wz9 3IPZ ENSEMBLE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.484 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.720 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.820 |
| Number of reflections | 37552 | |
| <I/σ(I)> | 11.7 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M ACETATE PH 4.5, 2 M(NH4)2 SO4. |
