2YAB
Crystal structure of the autoinhibited form of mouse DAPK2 in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.140, 85.370, 123.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.685 - 1.900 |
| R-factor | 0.1739 |
| Rwork | 0.172 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a2a |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.710 |
| Number of reflections | 53144 | |
| <I/σ(I)> | 15.2 | 2.3 |
| Completeness [%] | 99.3 | 97.5 |
| Redundancy | 7.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
