2YAA

Crystal structure of the autoinhibited form of mouse DAPK2 in complex with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	63.100, 86.020, 124.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.855 - 2.300
R-factor	0.2186
Rwork	0.216
R-free	0.27070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a2a
RMSD bond length	0.004
RMSD bond angle	0.767
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	0.480
Number of reflections	30082
<I/σ(I)>	17.4	4.2
Completeness [%]	97.6	97.8
Redundancy	7.1	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1