2YA0
Catalytic Module of the Multi-modular glycogen-degrading pneumococcal virulence factor SpuA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.574, 75.196, 87.038 |
| Unit cell angles | 90.00, 97.31, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.17452 |
| Rwork | 0.172 |
| R-free | 0.22662 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fhf |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.927 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 57697 | |
| <I/σ(I)> | 10.2 | 2.3 |
| Completeness [%] | 96.7 | 94.1 |
| Redundancy | 3.73 | 3.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
