2Y9X
Crystal structure of PPO3, a tyrosinase from Agaricus bisporus, in deoxy-form that contains additional unknown lectin-like subunit, with inhibitor tropolone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.840, 104.820, 119.360 |
| Unit cell angles | 90.00, 110.45, 90.00 |
Refinement procedure
| Resolution | 58.030 - 2.780 |
| R-factor | 0.238 |
| Rwork | 0.235 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y9w |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.950 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 2.930 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.240 | 0.620 |
| Number of reflections | 54004 | |
| <I/σ(I)> | 3.5 | 1.6 |
| Completeness [%] | 89.6 | 89.7 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 10% PEG 4000, 100 MM NA ACETATE PH 4.6, 5 MM HOLMIUM CHLORIDE. |
