2Y8U
A. nidulans chitin deacetylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.640, 64.520, 86.540 |
| Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
| Resolution | 84.900 - 1.990 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iw0 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.939 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.400 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.080 | 0.320 |
| Number of reflections | 26431 | |
| <I/σ(I)> | 10.3 | 3.9 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M NAH2PO4, 5% HEXANEDIOL, 20% PEG 3350 |
