2Y8S
Co-structure of an AMA1 mutant (Y230A) with a surface exposed region of RON2 from Toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.520, 96.830, 78.330 |
| Unit cell angles | 90.00, 116.75, 90.00 |
Refinement procedure
| Resolution | 56.700 - 2.550 |
| R-factor | 0.18908 |
| Rwork | 0.185 |
| R-free | 0.26252 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x2z |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.695 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.970 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 30766 | |
| <I/σ(I)> | 11.9 | 4.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG 1500, 100 MM MIB PH 4.0. |
