2Y8I
Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-28 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 55.000, 105.800, 180.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.215 - 3.132 |
R-factor | 0.2494 |
Rwork | 0.245 |
R-free | 0.33650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mmd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.360 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.220 | 3.190 |
High resolution limit [Å] | 3.130 | 3.130 |
Rmerge | 0.170 | 0.460 |
Number of reflections | 20093 | |
<I/σ(I)> | 9.23 | 2.37 |
Completeness [%] | 96.4 | 83.4 |
Redundancy | 11.1 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 13% PEG 6000, 4% GLYCEROL., pH 7.0 |