2Y7G
Crystal structure of the 3-keto-5-aminohexanoate cleavage enzyme (Kce) from C. Cloacamonas acidaminovorans in complex with the product acetoacetate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-05-22 |
Detector | ADSC CCD |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 102.100, 102.100, 100.736 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.000 - 1.400 |
R-factor | 0.1676 |
Rwork | 0.167 |
R-free | 0.18630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y7d |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.9.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.070 | 0.500 |
Number of reflections | 104015 | |
<I/σ(I)> | 12.5 | 3 |
Completeness [%] | 99.3 | 98.5 |
Redundancy | 7.2 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 18% PEG3350, 0.220 M MG FORMATE, pH 7.0 |