2Y3R
Structure of the tirandamycin-bound FAD-dependent tirandamycin oxidase TamL in P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-11-20 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.088, 106.171, 151.172 |
| Unit cell angles | 90.00, 94.63, 90.00 |
Refinement procedure
| Resolution | 150.680 - 1.790 |
| R-factor | 0.14969 |
| Rwork | 0.146 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y08 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.820 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.060 | 0.240 |
| Number of reflections | 161395 | |
| <I/σ(I)> | 10.6 | 2.4 |
| Completeness [%] | 76.7 | 26.9 |
| Redundancy | 2.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 296 | 9% PEG 4000, 0.2 M MGCL, 23 DEGREES CELSIUS., pH 7.0 |
